1. H&E/PAS slide scoring: subjective vs. objective?

2. Objective enables reproducibility

Starting from 2025-06-01, several Nature Portforlio journals (i.e. Nature Communications, Nature Cell Biology, Nature Methods, and Nature Structural & Molecular Biology) would ask authors to finish a reporting table summarizing key aspects, including image collection and image processing of light microscopy experiments, at revision stage1.

  • The reporting table will be shared with reviewers.

  • The reporting table will be published as a type of supplementary information.

  • The reporting table contains five sections describing the following information:

    1. Hardware.

    2. Quality control.

    3. Methodology.

    4. Acquisition.

    5. Imaging processing: software used for analysis and any workflows or parameters applied to the image data presented.

The Nature Portforlio journals also:

encourage authors to deposit their microscopy datasets in public repositories, as is routinely expected in other fields, such as genomics and proteiomics1.

GeneCard-BioImage believes rational image-relevant experiment design (wet lab work) and objective image analysis (dry lab work) are two important factors for reproducibility.

3. Subjective vs. objective? (for fun😂)

# The value of "objective"
objective <- "Objective" |> utf8ToInt() |> sum() * -1 
cat("The value of 'objective':", objective, "\n") 
The value of 'objective': -923 
# The value of "subjective"
subjective <- "Subjective" |> utf8ToInt() |> sum() * -1 
cat("The value of 'subjective':", subjective, "\n") 
The value of 'subjective': -1044 
# Subjective - objective
result <- subjective - objective 
cat("The result of 'subjective - objective':", result, "\n") 
The result of 'subjective - objective': -121 
# Conclusion
conclusion <- ifelse(result > 0, "Conclusion：'Subjective' is better than 'Objective' because 'Subjective' is larger~", "Conclusion: 'Objective' is better than 'Subjective' because 'Objective' is larger~")
cat(conclusion, "\n")
Conclusion: 'Objective' is better than 'Subjective' because 'Objective' is larger~